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primary human coronary artery vsmc  (PromoCell)


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    PromoCell primary human coronary artery vsmc
    Primary Human Coronary Artery Vsmc, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human coronary artery vsmc/product/PromoCell
    Average 93 stars, based on 104 article reviews
    primary human coronary artery vsmc - by Bioz Stars, 2026-03
    93/100 stars

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    Diabetic patient vSMCs exhibit DNA damage and low SIRT1 expression. ( A ) Double stranded DNA breaks were assessed via the comet assay in control ( n = 6) and diabetic (DM) ( n = 6) vSMCs. Tail moment and tail DNA percentage were calculated via CASPLab. Scale bar = 20μm. ( B ) Telomere length of control ( n = 6) and DM ( n = 6) vSMCs was quantified via qPCR and normalised to Col3a1 and Col6a1. ( C ) p21 ( n = 6) ( D ) p53, ( n = 6) ( E ) ATM ( n = 6) and ( F ) SIRT1 ( n = 6) mRNA expression from DM vSMCs was compared to controls via qPCR. ( G ) SIRT1 protein expression within DM and control vSMCs was assessed by western blot analysis and normalized to α-tubulin ( n = 6). A Mann–Whitney U test was performed. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Cardiovascular Research

    Article Title: Loss of SIRT1 in diabetes accelerates DNA damage-induced vascular calcification

    doi: 10.1093/cvr/cvaa134

    Figure Lengend Snippet: Diabetic patient vSMCs exhibit DNA damage and low SIRT1 expression. ( A ) Double stranded DNA breaks were assessed via the comet assay in control ( n = 6) and diabetic (DM) ( n = 6) vSMCs. Tail moment and tail DNA percentage were calculated via CASPLab. Scale bar = 20μm. ( B ) Telomere length of control ( n = 6) and DM ( n = 6) vSMCs was quantified via qPCR and normalised to Col3a1 and Col6a1. ( C ) p21 ( n = 6) ( D ) p53, ( n = 6) ( E ) ATM ( n = 6) and ( F ) SIRT1 ( n = 6) mRNA expression from DM vSMCs was compared to controls via qPCR. ( G ) SIRT1 protein expression within DM and control vSMCs was assessed by western blot analysis and normalized to α-tubulin ( n = 6). A Mann–Whitney U test was performed. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Non-diabetic primary human coronary artery vSMCs were purchased from Caltag, UK.

    Techniques: Expressing, Single Cell Gel Electrophoresis, Western Blot, MANN-WHITNEY